DNA Vectors
Invitrogen™ Expressway™ Maxi Cell-Free E. coli Expression System
Uses efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4 to 6 hours
Invitrogen™ pGlow TOPO™ TA Expression Kit
Uses Cycle 3 GFP mutant as reporter protein for transcriptional activity
Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit
Designed to allow fusion of protein of interest to Cycle 3 GFP protein
Invitrogen™ DES™-Inducible Kit with pCoBlast
Small size (3.5kb) to improve DNA yields and increase subcloning efficiency
Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli
Facilitates five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway entry vector with ≥85% efficiency
Invitrogen™ Ni-NTA Purification System
Designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence
Invitrogen™ InsectSelect™ Glow Kit with Sf9 Cells
Features the pIZT/V5-His vector for high-level expression of the gene of interest
Invitrogen™ ProBond™ Purification System
Designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence
Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit
Offers five-minute cloning of Taq-amplified PCR products directly into the pIB/V5-His-TOPO expression vector
Invitrogen™ pEF6/V5-His TOPO™ TA Expression Kit
Designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO Cloning
Invitrogen™ Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression
Invitrogen™ DES™-Inducible/Secreted Kit with pCoHygro
For inducible, secreted expression of recombinant proteins and hygromycin (pCoHygro)
Invitrogen™ pTrcHis2 TOPO™ TA Expression Kit
Allow fast, efficient cloning of PCR products into prokaryotic expression vector for high-level, regulated expression from trc promoter
Invitrogen™ pTrcHis TOPO™ TA Expression Kit
Allow fast, efficient cloning of PCR products into prokaryotic expression vector for high-level, regulated expression from trc promoter
Invitrogen™ Gateway™ pcDNA™-DEST40 Vector
Designed for high-level, constitutive expression in variety of mammalian cell lines